| S Preparation
|
The glucose‐tolerant strain of Synechocyst … The glucose‐tolerant strain of Synechocystis sp. PCC 6803, isolated by Williams (1988), and the hik8‐overexpressing strain, designated as HOX80, were grown in modified BG‐11 medium, which consisted of BG‐110 liquid medium (Rippka, 1988) containing 5 mM NH4Cl (buffered with 20 mM Hepes‐KOH, pH 7.8). Among GT substrains, the GT‐I strain was used in this study (Kanesaki et al., 2012). Liquid cultures were bubbled with 1% (v/v) CO2 in air and incubated at 30°C under continuous white light (c. 50–70 μmol photons m−2 s−1). For plate cultures, modified BG‐11 medium (containing 10 mM NH4Cl in liquid medium) was solidified with agar (1.5% w/v), and cultures were incubated in air at 30°C under continuous white light (c. 50–70 μmol photons m−2 s−1). Growth and cell densities were measured at OD730 with a Hitachi U‐3310 spectrophotometer (Hitachi High‐Tech., Tokyo, Japan). ometer (Hitachi High‐Tech., Tokyo, Japan).
|
