| S Preparation
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Arabidopsis thaliana (ecotype Columbia) pl … Arabidopsis thaliana (ecotype Columbia) plants were used as the wild‐type plant in this study. The pap1‐D mutant was described previously (Borevitz et al., 2000). The PAP1 cDNA over‐expressing transformant was obtained by transformation of A. thaliana with the engineered Ti plasmid carrying cauliflower mosaic virus 35S promoter linked with the coding sequence of PAP1 cDNA. The plants were cultured on GM‐agar medium containing 1% sucrose (Valvekens et al., 1988) in a growth chamber at 22°C in 16/8 h light and dark cycles for 3 weeks, or in a standard greenhouse at 22°C in 16/8 h light for 4 weeks. Samples from wild‐type plant and PAP1over‐expressing lines were used, namely: WLA (wild‐type leaves grown on GM agar medium); PLA (pap1‐D mutant leaves grown on GM agar medium); OLA (PAP1‐over‐expressed transgenic leaves grown on GM agar medium); WLV (wild‐type leaves grown on vermiculite); PLV (pap1‐D mutant leaves grown on vermiculite); WRA (wild‐type roots grown on GM medium); and PRA (pap1‐D mutant roots grown on GM medium). The leaves and roots of plants were harvested, immediately frozen with liquid nitrogen and stored at −30°C until use. id nitrogen and stored at −30°C until use.
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