| MS Comment of details
|
[column] TSKgel-100V (4.6 x 250 mm, 5 micr … [column] TSKgel-100V (4.6 x 250 mm, 5 micrometer; TOSOH)
[gradient] Solvent: A; 0.1% formic acid aq. B; ACN (addition 0.1% formic acid fc.), Gradient: (B);3 to 30% (0.0 to 25.0 min), 30 to 90% (25.0 to 40.0 min), 90% (40.0 to 45.0 min), 95% (45.1 to 50.0 min), 3% (50.1 to 57.0 min)
[total separation time] 60 min o 57.0 min)
[total separation time] 60 min
|
| MS Description
|
Harvested sample is frozen by liquid N2 an … Harvested sample is frozen by liquid N2 and resulting powder (100mg) are solved in 300uL 80% methanol solution. 20uL sample is injected into HPLC after 0.2um membrane filter treatment. HPLC conditions: Agilent 1100 series (Agilent), Column: TSKgel-100V (4.6 x 250 mm, 5 micrometer; TOSOH), Solvent: A; 0.1% formic acid aq. B; ACN (addition 0.1% formic acid fc.), Gradient: (B);3 to 30% (0.0 to 25.0 min), 30 to 90% (25.0 to 40.0 min), 90% (40.0 to 45.0 min), 95% (45.1 to 50.0 min), 3% (50.1 to 57.0 min), Column temp.: 30 degree C, Flow rate=0.5mL/min, PDA: 200-650 nm (2 nm step). FT-ICR-MS conditions: Filter 1: FTMS + c norm !corona !pi res=100000 o(200.0-1500.0); 2: ITMS + c norm !corona !pi Dep MS/MS Most intense ion from (1)., Rejected mass=256.2000;266.0000;284.2000;300.2000;328.2000;344.2000;372.2000;388.2000;416.3000;432.3000;460.0000. .2000;388.2000;416.3000;432.3000;460.0000.
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| MS ID
|
MS1 +
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| MS Instrument
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Agilent1100 HPLC (Agilent), LTQ-FT (Thermo Fisher Scientific) +
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| MS Instrument Type
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LC-FTICR-MS +
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| MS Ion Mode
|
Positive +
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| MS Ionization
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ESI +
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| MS Title
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LC-FT-ICR-MS ESI positive method 1 +
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| Modification dateThis property is a special property in this wiki.
|
15 December 2016 06:03:25 +
|
