Browse wiki

jump-to-nav Jump to: navigation, search
SE28:/MS2
MS Description Harvested sample is frozen by liquid N2 an Harvested sample is frozen by liquid N2 and resulting powder (100mg) are solved in 300uL 80% methanol solution. 20uL sample is injected into HPLC after 0.2um membrane filter treatment. HPLC conditions: Agilent 1100 series (Agilent), Column: TSKgel-100V (4.6 x 250 mm, 5 micrometer; TOSOH), Solvent: A; 0.1% formic acid aq. B; ACN (addition 0.1% formic acid fc.), water used for mobile phase is purchased from Wako Co., Gradient: (B);3 to 30% (0.0 to 25.0 min), 30 to 90% (25.0 to 40.0 min), 90% (40.0 to 45.0 min), 95% (45.1 to 50.0 min), 3% (50.1 to 57.0 min), Column temp.: 40 degree C, Flow rate=0.5mL/min, PDA: 200-650 nm (2 nm step). FT-ICR-MS conditions: Filter 1: FTMS + c norm !corona !pi res=100000 o(100.0-1500.0); 2: ITMS + c norm !corona !pi Dep MS/MS Most intense ion from (1).; 3: ITMS + c norm !corona !pi Dep MS/MS 2nd most intense ion from (1)., Rejected mass=224.1900, 225.1930, 235.1800, 236.1830, 266.2300, 267.2330, 294.2600, 295.2630, 308.0000, 309.0000, 310.0000, 311.0000, 311.9900, 375.9000, 376.9000, 377.9000, 378.0400, 380.0300, 381.0400, 609.2800, 610.2900, 611.2900, 780.5500, 784.5900, 785.5900, 786.5900, 810.7000, 811.7000, 1101.6900, 1102.7000, 1123.6800, 1124.6830, 1125.6800. 102.7000, 1123.6800, 1124.6830, 1125.6800.
MS ID MS2  +
MS Instrument Agilent1100 HPLC (Agilent), LTQ-FT (Thermo Fisher Scientific)  +
MS Instrument Type LC-FTICR-MS  +
MS Ion Mode Positive  +
MS Ionization ESI  +
MS Title LC-FT-ICR-MS ESI positive method 2  +
Modification dateThis property is a special property in this wiki. 23 April 2022 17:16:20  +
hide properties that link here 
  No properties link to this page.
 

 

Enter the name of the page to start browsing from.
Personal tools
View and Edit Metadata
Variants
Views
Actions
Toolbox