Browse wiki

jump-to-nav Jump to: navigation, search
SE44:/MS3
MS Comment of details 20130424_Standerd_ESp.meth
MS Description Harvested sample is frozen by liquid N2 an Harvested sample is frozen by liquid N2 and resulting powder (100mg) are solved in 300uL 80% methanol solution. 5uL sample is injected into HPLC after 0.2um membrane filter treatment. HPLC conditions: Agilent 1200 series (Agilent), Column: TSKgel ODS-100V (4.6 x 250 mm, 5 micrometer; TOSOH), Solvent: A; 0.1% formic acid aq. B; ACN (addition 0.1% formic acid fc.), Gradient: (B) 3 to 97% for 45 min, 97% for 5 min and 97 to 3% for 7 min, Column temp.: 40 degree C, Flow rate=0.5mL/min, PDA: 190-950 nm (2 nm step). Orbitrap-MS conditions: Filter 1: FTMS + c norm !corona !pi res=60000 o(100.0-1500.0); 2: ITMS + c norm !corona !pi Dep MS/MS Most intense ion from (1); 3: ITMS + c norm !corona !pi Dep MS/MS 2nd most intense ion from (1); 4: ITMS + c norm !corona !pi Dep MS/MS 3rd most intense ion from (1); 5: ITMS + c norm !corona !pi Dep MS/MS 4th most intense ion from (1); 6: ITMS + c norm !corona !pi Dep MS/MS 5th most intense ion from (1). Reject Mass List:102.09139, 110.02006, 141.95860, 158.00286, 158.96135, 163.13289, 171.14921, 182.98517, 186.95628, 195.08767, 199.98790, 207.15909, 223.06365, 224.18561, 227.98281, 232.98011, 235.18048, 249.20600, 266.23260. 32.98011, 235.18048, 249.20600, 266.23260.
MS ID MS3  +
MS Instrument Agilent1200 HPLC (Agilent), LTQ-Orbitrap (Thermo Fisher Scientific)  +
MS Instrument Type LC-Orbitrap-MS  +
MS Ion Mode Positive  +
MS Ionization ESI  +
MS Title LC-Orbitrap-MS ESI positive method 3  +
Modification dateThis property is a special property in this wiki. 1 June 2020 06:21:22  +
hide properties that link here 
  No properties link to this page.
 

 

Enter the name of the page to start browsing from.
Personal tools
View and Edit Metadata
Variants
Views
Actions
Toolbox