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Seeds of transposon-tagged mutants were an … Seeds of transposon-tagged mutants were analyzed for metabolic profiling. From the Ds transposon singlecopy insertion lines that we previously established (Kuromori et al. 2006), we used 2,656 knockout mutants in which the Ds transposon was homozygously inserted into the coding regions. For each gene mutant, seeds were harvested from an F3 individual plant described in Kuromori et al. (2006). Briefly, F3 seeds were sown on agarsolidified MS medium containing 3% w/v sucrose and 20 lg mL-1 hygromycin. After stratification at 4℃ for 1 week, plants were grown for 3 weeks under a 16-h light/8-h dark cycle at 22℃, transferred to soil and grown under the same conditions with application of 2,000-fold diluted Hyponex (Hyponex Japan Co Ltd., Osaka, Japan) until seed maturity. o Ltd., Osaka, Japan) until seed maturity.
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