| MS Description
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Tissue processing method: The lyophilized … Tissue processing method: The lyophilized materials were ground using a mixer mill (MM300, Retsch) with zirconia beads for 2 min at 18 Hz and 4 °C.
Storage condition prior to extraction or further processing: The lyophilized powder materials were stored at −80 °C.
Extraction method: The freeze-dried samples were extracted with 50 ml of 80% MeOH containing 2.5 mM lidocaine per mg dry weight using a mixer mill (MM300, Retsch) with zirconia beads for 10 min at 20 Hz and 4 °C.
Extract cleanup and/or Additional manipulation: After centrifugation for 10 min, the supernatant was filtered using an HLB mElution plate (Waters).
Extract storage and/or relocate: not storaged
Chromatography instrument description: LC, Waters Acquity UPLC system
Separation column and pre/guard column: column, Acquity BEH C18 (1.7 μm, 2.1 mm x 100 mm, Waters)
Separation parameter: solvent system, solvent A (water with 0.1% formic acid) and solvent B (acetonitrile with 0.1% formic acid)
Instrument description: Waters Xevo G2 Q-Tof
Sample introduction and delivery: From LC
Ionization source: ESI positive. column temperature, 40 °C; MS detection: capillary voltage, +3.0 keV, cone voltage, 25.0 V, source temperature, 120 °C, desolvation temperature, 450 °C, cone gas flow, 50 l/h; desolvation gas flow, 800 l/h; collision energy, 6 V; MS/MS detection: collision energy, rampled from 10 to 50V
Mass analyzer description and acquisition mode: quadrupole-time-of-flight
Data acquisition parameters: MS: mass range, m/z 100‒1500; scan duration, 0.1 sec; interscan delay, 0.014 sec; data acquisition, centroid mode. MS/MS: m/z 50–1500; scan duration, 0.02 sec; inter-scan delay, 0.014 sec; data acquisition, centroid mode; MS/MS spectra of the top 10 ions (> 1000 counts) in an MS scan were automatically obtained. in an MS scan were automatically obtained.
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