| S Preparation
|
BioSource Species<br />
Soybean Glyc … BioSource Species<br />
Soybean Glycine max (9 varieties)<br />
<br />
Genotypes/Varieties<br />
Williams, A3127, A3469, A3555, A3733/CX329 (CX375), AG3701, AG3803, CX366, and AG3705<br />
<br />
Organ specification<br />
Mature seeds<br />
<br />
Growth conditions<br />
Nine soybean varieties representing a genetic lineage from Williams (1972) to A3555 (2008) were grown at two sites in Illinois (Jerseyville [ILJA] and Jacksonville [ILJA]) during the 2011 season. Varieties included six conventional and three glyphosate-tolerant lines. Starting seeds were planted in a randomized complete block design with six replicates. Soybean plants were treated with maintenance pesticides as necessary throughout the growing season at both sites. The three Roundup Ready varieties were not treated with glyphosate. <br />
<br />
Experimental conditions<br />
Same as the growth conditions. Soybean seeds of 5-6 biological replications were harvested at maturity on 2011. Seeds for each replicate was homogenized by grinding with dry ice to a fine powder, lyophilized and stored frozen at approximately -20°C prior to analysis. We weighed 70 mg dry weight (DW) for CE-TOF-MS analysis, 5 mg DW for GC-TOF-MS analysis, 50 mg DW for LC-q-TOF-MS analysis to detect polar metabolites, and 15 mg DW for lipid profiling. <br /> 15 mg DW for lipid profiling. <br />
|
