SE129:/S3
From Metabolonote
Sample Set Information
| ID | TSE9 |
|---|---|
| Title | MS-DIAL demo files |
| Description | Both data independent MS/MS acquisition (SWATH) and data dependent MS/MS acquisition (IDA) data sets is downloaded as the demo files of MS-DIAL. In order to use MS-DIAL program, the user has to convert the vendor's raw data to ABF file format. The demonstration for file convert can be performed via AB Sciex raw data sets (.wiff and .wiff.scan). The file converter is available at http://www.reifycs.com/english/AbfConverter/. If you want to demonstrate MS-DIAL itself, please use the converted files (.abf) from the below link. Also see http://prime.psc.riken.jp/Metabolomics_Software/MS-DIAL/MSDIAL%20quick%20start.pdf |
| Authors | Hiroshi Tsugawa, Tomas Cajka, Tobias Kind, Yan Ma, Brendan Higgins, Kazutaka Ikeda, Mitsuhiro Kanazawa, Jean VanderGheynst, Oliver Fiehn & Masanori Arita |
| Reference | Tsugawa et al. (2015) Nature Methods 12(6):523–526 |
| Comment |
The raw data files are available at DROP Met web site in PRIMe database of RIKEN.
Sample Information
| ID | S3 |
|---|---|
| Title | Chlorella sorokiniana (UTEX 2805) |
| Organism - Scientific Name | Chlorella sorokiniana |
| Organism - ID | NCBI taxonomy 3076 |
| Compound - ID | |
| Compound - Source | |
| Preparation | UTEX 2341 (originally classified as Chlorella minutissima), Chlorella sorokiniana (UTEX 2805), and Chlorella variabilis (ATCC NC64A) were plated on ATCC #5 agar and colonies were selected for inoculation into liquid cultures. All three Chlorella strains were cultivated simultaneously in 250 mL hybridization tubes with four independent cultures per strain. Hybridization tubes were filled with 200 mL media and maintained in a 28 °C water bath. Aeration was supplied at 125 mL per minute with 2% CO2 mixed with air (v/v). Reactors were illuminated horizontally (10,000 lx) by T5 growth lamps operating on a 16:8 light/dark cycle and cultures were mixed by stir bar operating at ∼150 rpm. UTEX 2341 was cultivated in N8-NH4 medium30, C. sorokiniana in N8 medium31 and C. variabilis in N8-NH4 medium supplemented with 20 mg/L yeast extract. Culture samples (1 mL) were quenched for lipidomics analysis during the late log growth 00stage. |
| Sample Preparation Details ID | |
| Comment | Tsugawa et al. (2015) Nature Methods 12(6):523–526 |
