SE134:/S1
Sample Set Information
ID | SE134 |
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Title | Unbiased profiling of volatile organic compounds in the headspace of Allium plants using an in-tube extraction device. |
Description | Plants produce and emit important volatile organic compounds (VOCs), which have an essential role in biotic and abiotic stress responses and in plant–plant and plant–insect interactions. In order to study the bouquets from plants qualitatively and quantitatively, a comprehensive, analytical method yielding reproducible results is required. We applied in-tube extraction (ITEX) and solid-phase microextraction (SPME) for studying the emissions of Allium plants. The collected HS samples were analyzed by gas chromatography–time-of-flight–mass spectrometry (GC-TOF–MS), and the results were subjected to multivariate analysis. In case of ITEX-method Allium cultivars released more than 300 VOCs, out of which we provisionally identified 50 volatiles. We also used the VOC profiles of Allium samples to discriminate among groups of A. fistulosum, A. chinense (rakkyo), and A. tuberosum (Oriental garlic). As we found 12 metabolite peaks including dipropyl disulphide with significant changes in A. chinense and A. tuberosum when compared to the control cultivar, these metabolite peaks can be used for chemotaxonomic classification of A. chinense, tuberosum, and A. fistulosum. |
Authors | Kusano M, Kobayashi M, Iizuka Y, Fukushima A, Saito K. |
Reference | BMC Res Notes. 2016 Feb 29;9:133. doi: 10.1186/s13104-016-1942-5. |
Comment |
Sample Information
ID | S1 |
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Title | Allium fistulosum species |
Organism - Scientific Name | Allium fistulosum L. |
Organism - ID | NCBI taxonomy:35875 |
Compound - ID | |
Compound - Source | |
Preparation | Ten Allium (A.) fistulosum species, six spring onion cultivars, two scallions, and two Japanese-leek cultivars, rakkyo (A. chinense) and Oriental garlic (A. tuberosum), were purchased from a grocer in Kawasaki, Japan or harvested in a Japanese field (see Table 1 and Additional file 2). After removing the roots, a 10-cm length of the sheath and the basal plate of each plant sample were collected and chopped with stainless steel surgical blades (Feather, Tokyo, Japan). Out of the A. fistulosum cultivars, four were grown by applying a method (hilling) similar to that used for growing the leek A. ampeloprasum var. porrum to obtain longer white stems for consumption in Japan (Fig. 1f, g, h, l). Each sample was immediately frozen in liquid nitrogen and kept at −80 °C until use. As the group of samples of Mikata spring onion (class01) was gathered center of the PCA score scatter plot (Fig. 4), this cultivar was chosen as the control. |
Sample Preparation Details ID | |
Comment |
Class in PCA |
Binomial name |
Species name |
Bland name | Harvested field in Japan | |
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08 | Allium chinense |
Rakkyo | Young rakkyo | Namegata,Ibaraki | |
01 | Allium fistulosum |
Spring onion | Mikata spring onion | Hamamatsu, Shizuoka |
|
02 | Allium fistulosum |
Green spring onion |
Aoi-chan green spring onion | Akitakata, Hiroshima |
|
03 | Allium fistulosum |
Scallion | Hakata scallion | Hakata, Fukuoka | |
04 | Allium fistulosum |
Green spring onion |
Green spring onion from Nagareyama chiba |
Nagareyama, Chiba | |
05 | Allium fistulosum |
White spring onion |
White spring onion from Nagano |
Nagano | |
06 | Allium fistulosum |
Leek | Shimonita leek | Gunma | |
07 | Allium fistulosum |
Leek | Shirakami leek | Noshiro, Akita | |
09 | Allium fistulosum |
Scallion | Kujo scallion | Nagahama, Gifu | |
11 | Allium fistulosum |
Spring onion | Goudo spring onion | Anpachi, Gifu | |
10 | Allium tuberosum |
Oriental garlic | Oriental garlic | Nagahama, Shiga | |
12 | Allium fistulosum |
Red spring onion |
Red spring onion | Tsuruoka, Yamagata |