SE15:/S01/M05

Sample Set Information

ID	SE15
Title	Comparison of fruit metabolites among developmental stages of Jatropha curcas
Description	Investigation of Jatropha curcas fruit metabolites. 4 developmental stages and 3 replicates data are examined.
Authors	Ryosuke Sano 1, Takeshi Ara 1, Nayumi Akimoto 1, Yoshihiko Morishita 1, Mitsuo Enomoto 1, Nozomu Sakurai 1, Hideyuki Suzuki 1, Yasunori Fukuzawa 2, Yoshinobu Kawamitsu 2, Masami Ueno 2, Daisuke Shibata 1, 1: Kazusa DNA Research Institute, 2: Faculty of Agriculture, University of the Ryukyus
Reference	Sano R. et al. (2012) Plant Biotechnology 29: 175-178
Comment	version 5

The web resources and information related to the species used in this study are available at Plant Genome Database Japan (PGDBj).

http://pgdbj.jp/plantdb/plantinfo.html?ln=en&cmd=entry&ppid=t180498

The original paper is available at Plant Biotechnology

Sample Information

ID	S01
Title	Jatropha curcas Fruit stage1 (female flower-buds)
Organism - Scientific Name	Jatropha curcas
Organism - ID	NCBI taxonomy:180498
Compound - ID
Compound - Source
Preparation	Jatropha curcas are grown in an open experimental　field at the University of the Ryukyus (Okinawa island, Okinawa, Japan), located in a subtropical area.
Sample Preparation Details ID
Comment	[KomicMarket ID] KSBA_3

Analytical Method Information

ID	M05
Title	LC-Orbitrap-MS, ESI Negative analysis
Method Details ID	MS2
Sample Amount
Comment	[MassBase ID] MDLC1_25035

The raw (binary) and near-raw (text) files of this analysis are available at MassBase.

Analytical Method Details Information

ID	MS2
Title	LC-Orbitrap-MS ESI negative method 1
Instrument	Agilent1100 HPLC (Agilent), LTQ-Orbitrap (Thermo Fisher Scientific)
Instrument Type	LC-Orbitrap-MS
Ionization	ESI
Ion Mode	Negative
Description	Harvested sample is frozen by liquid N2 and ground to powder by a homogenizer, Shake Master (Biomedical Science, Tokyo, Japan). The powdered sample (100mg) are solved in 300uL 80% methanol solution. 10uL sample is injected into HPLC after 0.2um membrane filter treatment. HPLC conditions: Agilent 1100 series (Agilent), Column: TSKgel ODS-100V (4.6 x 250 mm, 5 micrometer; TOSOH), Solvent: A; 0.1% formic acid aq. B; ACN (addition 0.1% formic acid fc.), Gradient: (B);3 to 97% (0.0 to 45.0 min), 97% (45.1 to 50.0 min), 3% (50.1 to 57.0 min), Column temp.: 40 degree C, Flow rate=0.5mL/min, PDA: 200-650 nm (2 nm step). Orbitrap-MS conditions: Filter 1: FTMS - c norm !corona !pi res=60000 o(100.0-1500.0); 2: ITMS - c norm !corona !pi Dep MS/MS Most intense ion from (1); 3: ITMS - c norm !corona !pi Dep MS/MS 2nd most intense ion from (1); 4: ITMS - c norm !corona !pi Dep MS/MS 3rd most intense ion from (1); 5: ITMS - c norm !corona !pi Dep MS/MS 4th most intense ion from (1); 6: ITMS - c norm !corona !pi Dep MS/MS 5th most intense ion from (1).
Comment_of_details