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Sample Set Information

ID TSE1307
Title Induced accumulation of glucuronosyldiacylglycerol in tomato and soybean under phosphorus deprivation.
Description Glucuronosyldiacylglycerol (GlcADG) is a plant glycolipid that accumulates in Arabidopsis and rice in response to phosphorus (P) starvation. It has been suggested that GlcADG functions to mitigate the stress induced by P depletion. Biosynthesis of GlcADG requires sulfolipid (SQDG) synthase, which is coded for in plant genomes. This indicates the possibility that GlcADG may be a general constituent of membrane lipids in plants. In this study, we investigated the SQDG synthases found in the genomes of higher plants, ferns, mosses, algae and cyanobacteria. In addition, we analyzed GlcADG accumulation, and the expression of SQDG synthase homologs in tomato and soybean plants grown under P-limited conditions. LC-MS analysis of lipids from these plants confirmed that GlcADG accumulated during P deprivation, as previously observed in Arabidopsis and rice. We also observed upregulation of SQDG synthase transcripts in these plants during P deprivation. These data suggest that GlcADG is present not only in model plants, but also in various other plant species, and that this lipid molecule performs an important physiological function as a mitigator of P-deprivation stress in plants.
Authors Okazaki Y, Nishizawa T, Takano K, Ohnishi M, Mimura T, Saito K.
Reference Physiol Plant. 2015 Sep;155(1):33-42. doi: 10.1111/ppl.12334. Epub 2015 Mar 12.

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Sample Information

Title Soybean
Organism - Scientific Name Glycine max
Organism - ID NCBI taxonomy:3847
Compound - ID
Compound - Source
Preparation Soybean seeds (Glycine max (L.) Merr. cv. Enrei) were agitated in 2% PPM solution, and sown in wet potting mix consisting of 2:1 (v/v) PRO-MIX BX (Premier Tech Horticulture, Rivière-du-Loup (Québec), Canada) and vermiculite (VS kakou, Minato-ku, Tokyo, Japan), and grown under a 28°C/20°C day/night temperature cycle with a 12 h photoperiod. The roots of 10-day-old soybean plants were gently washed to remove the soil, then transferred to rockwool, where the P levels were controlled as described above.
Sample Preparation Details ID

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