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Sample Set Information

ID TSE1307
Title Induced accumulation of glucuronosyldiacylglycerol in tomato and soybean under phosphorus deprivation.
Description Glucuronosyldiacylglycerol (GlcADG) is a plant glycolipid that accumulates in Arabidopsis and rice in response to phosphorus (P) starvation. It has been suggested that GlcADG functions to mitigate the stress induced by P depletion. Biosynthesis of GlcADG requires sulfolipid (SQDG) synthase, which is coded for in plant genomes. This indicates the possibility that GlcADG may be a general constituent of membrane lipids in plants. In this study, we investigated the SQDG synthases found in the genomes of higher plants, ferns, mosses, algae and cyanobacteria. In addition, we analyzed GlcADG accumulation, and the expression of SQDG synthase homologs in tomato and soybean plants grown under P-limited conditions. LC-MS analysis of lipids from these plants confirmed that GlcADG accumulated during P deprivation, as previously observed in Arabidopsis and rice. We also observed upregulation of SQDG synthase transcripts in these plants during P deprivation. These data suggest that GlcADG is present not only in model plants, but also in various other plant species, and that this lipid molecule performs an important physiological function as a mitigator of P-deprivation stress in plants.
Authors Okazaki Y, Nishizawa T, Takano K, Ohnishi M, Mimura T, Saito K.
Reference Physiol Plant. 2015 Sep;155(1):33-42. doi: 10.1111/ppl.12334. Epub 2015 Mar 12.

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Sample Information

Title Chara australis
Organism - Scientific Name Chara australis
Organism - ID NCBI taxonomy:31298
Compound - ID
Compound - Source
Preparation Chara australis was cultured in plastic tanks on a substrate of garden soil and tap water at room temperature (25°C) under fluorescent lamps, on a 16-h light/8-h dark cycle. Internodal cells were excised from shoots and stored in artificial pond water (APW), composed of 1mM NaCl, 0.1 mM KCl and 0.5 mM CaCl2 (Mimura et al. 1998), for at least one night prior to the experiment. In order to generate P-deficient and P-enriched cells, the excised internodal cells were incubated in either APW alone, or APW supplemented with 0.1 mM sodium dihydrogen phosphate, respectively, for 1 week. The incubation media were exchanged twice each day.
Sample Preparation Details ID

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