SE16:/S08/M02/D03

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Sample Set Information

ID SE16
Title Effect of agricultural films for spinach leaf metabolites 2
Description Investigation of Spinacia oleracea leaf metabolites. 3 growth conditions (covered with normal or UV cut agricultural film or no agricultural film), 4 cultivers (Kanaji, Kurohaminstarland, Nihon and ,Wasesaradaakari) and 3 replicates data are examined.
Authors Takeshi Ara 1, Naoki Yamamoto 1, Yoshiki Nagashima 1, Kunihiro Suda 1, Mitsuo Enomoto 1, Nozomu Sakurai 1, Hideyuki Suzuki 1, Tatsuya Suzuki 2, Daisuke Shibata 1, 1: Kazusa DNA Research Institute, 2: Chiba Prefectural Agriculture Research Center
Reference Direct Submittion
Comment version 1


Link icon database.png Link icon pgdbj.png

The web resources and information related to the species used in this study are available at Plant Genome Database Japan (PGDBj).

http://pgdbj.jp/plantdb/plantinfo.html?ln=en&cmd=entry&ppid=t3562

Sample Information

ID S08
Title Spinacia oleracea Kanaji Leaf
Organism - Scientific Name Spinacia oleracea
Organism - ID NCBI taxonomy:3562
Compound - ID
Compound - Source
Preparation Spinacia oleracea are grown at agricultural field in natural conditions under UV cut film.
Sample Preparation Details ID
Comment [KomicMarket ID] KSBA_60

Analytical Method Information

ID M02
Title LC-FTICR-MS, ESI Positive analysis
Method Details ID MS1
Sample Amount 6.7mg
Comment [MassBase ID] MDLC1_35970


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The raw (binary) and near-raw (text) files of this analysis are available at MassBase.

Analytical Method Details Information

ID MS1
Title LC-FT-ICR-MS ESI positive method 1
Instrument Agilent1100 HPLC (Agilent), LTQ-FT (Thermo Fisher Scientific)
Instrument Type LC-FTICR-MS
Ionization ESI
Ion Mode Positive
Description Harvested sample is frozen by liquid N2 and resulting powder (100mg) are solved in 300uL 80% methanol solution. 20uL sample is injected into HPLC after 0.2um membrane filter treatment. HPLC conditions: Agilent 1100 series (Agilent), Column: TSKgel-100V (4.6 x 250 mm, 5 micrometer; TOSOH), Solvent: A; 0.1% formic acid aq. B; ACN (addition 0.1% formic acid fc.), Gradient: (B);3 to 97% (0.0 to 45.0 min), 97% (45.1 to 50.0 min), 3% (50.1 to 57.0 min), Column temp.: 40 degree C, Flow rate=0.5mL/min, PDA: 200-650 nm (2 nm step). FT-ICR-MS conditions: Filter 1: FTMS + c norm !corona !pi res=100000 o(100.0-1500.0); 2: ITMS + c norm !corona !pi Dep MS/MS Most intense ion from (1); 3: ITMS + c norm !corona !pi Dep MS/MS 2nd most intense ion from (1); 4: ITMS + c norm !corona !pi Dep MS/MS 3rd most intense ion from (1); 5: ITMS + c norm !corona !pi Dep MS/MS 4th most intense ion from (1); 6: ITMS + c norm !corona !pi Dep MS/MS 5th most intense ion from (1)., Rejected mass=235.1800, 376.0400, 378.0400, 540.6000, 609.2800, 726.3600, 810.4200, 818.4200, 1118.7200, 1123.6800.
Comment_of_details


link=SE12_MS1

The data can be compared with the data obtained by the method SE12_MS1

Data Analysis Information

ID D03
Title PowerGet data analysis for KomicMarket
Data Analysis Details ID DS3
Recommended decimal places of m/z 6|ITMS 2
Comment [KomicMarket ID] KSBA_60


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The annotation data and quantitative data of the metabolite peaks in this sample are available at KomicMarket.

Data Analysis Details Information

ID DS3
Title PowerGet analysis for detection of all peaks (C3)
Description Raw data files are converted to text file by MSGet software without cut off value and peaks are extracted from the text files by PowerFT with parameters (intensity cut off=5000, peak selection filter is default, intensity cut off in peak assignment=1000).
Comment_of_details
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