SE204:/S03

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Sample Set Information

ID SE204
Title Untargeted metabolome analysis of tobacco (BY-2) cells using LC-MS / LC-MSを用いたタバコ(BY-2)培養細胞のメタボローム解析
Description Untargeted metabolome analyses of tobacco (BY-2) cell cultures using liquid chromatography-mass spectrometry (LC-MS).

The information on the peaks detected in this study was available at the Plant Metabolome Repository website (http://metabolites.in/plants).


液体クロマトグラフィー-質量分析(LC)を用いたタバコ(BY-2)培養細胞のノンターゲットメタボローム解析です。

検出されたピークは、植物メタボロームレポジトリ (http://metabolites.in/plants) から公開されています。

Authors Sakurai N1,2, Suda K1, Masumoto H1, Kugou K1, Akimoto N1, Hoshi K1, Osawa S1, Ikeda C1, Ozawa K1, Yamada M1, Muneto R1, Shibata D1 (1 Kazusa DNA Research Institute, 2 National Institute of Genetics), Contact: sakurai AT nig.ac.jp (replace AT with @)


櫻井望1,2、須田邦裕1、桝本寛1、久郷和人1、秋元奈弓1、星久美1、大澤祥子1、池田千晶1、小澤馨史1、山田学1、宗藤玲子1、柴田大輔1(1 かずさDNA研究所, 2 国立遺伝学研究所)

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Sample Information

ID S03
Title BY2 cells, 3 days
Organism - Scientific Name Nicotiana tabacum
Organism - ID NCBI taxonomy: 4097
Compound - ID
Compound - Source
Preparation
Sample Preparation Details ID SS1
Comment


Sample Preparation Details Information

ID SS1
Title Preparation of the samples
Description The tobacco BY-2 cells were provided by the RIKEN BRC through the National Bio-Resource Project of MEXT, Japan. The cells were cultured according to a general protocol. The cells or culture media were sampled after 0, 3, or 7 days after inoculation. The samples were immediately frozen in liquid nitrogen, and stored at -80C. The cell sample was homogenated using mortar and pestle under liquid nitrogen into a fine powder and stored at -80C until use.
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