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Sample Set Information

Title Arabidopsis thaliana leaf metabolite analysis for a software test
Description Investigation of Arabidopsis thaliana leaf metabolites. The raw data file obtained from M01 was used one of the data files that were investigated to optimize the parameters of PowerGet software for better metabolite annotation in high-throughput metabolome analyses. The analysis data of D01 was annotated manually and was used as a reference to annotate peak data from different parameters. The analysis data of D01 was registered in KomicMarket.
Authors Takeshi Ara, Ryosuke Sasaki, Mitsuo Enomoto, Nozomu Sakurai, Hideyuki Suzuki, Daisuke Shibata, Kazusa DNA Research Institute
Reference Direct Submittion
Comment version 1

Link icon database.png Link icon pgdbj.png

The web resources and information related to the species used in this study are available at Plant Genome Database Japan (PGDBj).

Data Analysis Details Information

Title PowerGet analysis for detection of all peaks (C1)
Description Raw data files are converted to text file by MSGet software without cut off value and peaks are extracted from the text files by PowerFT with parameters (intensity cut off=10000, peak selection filter is default, peak shape is manually checked).
Comment_of_details At first, all peak shapes were manually selected by eye at both peak selection step and peak assignment step to make a reference data set. The data set was manually annotated after database search process. Then data sets made by different values (cut off intesity=0, 1000, 5000, 10000, 50000, 100000 in peak detection step) without manual check at peak selection step were manually annotated after database search process. The selected value (5000) showed >90% coverage of metabolite annotation of the reference data set.

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