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Sample Set Information

ID SE210
Title Comparison of seed metabolites among soybean varieties
Description Investigation of Glycine max seed metabolites. 3 cultivers (Koitozairai, Masshoku, Misuzu) and Glycine max subsp. soja and 7 or 8 replicates data are examined.
Authors Takeshi Ara 1, Yoshihiko Morishita 1, Hideyuki Suzuki 1, Kyuya Harada 2, Daisuke Shibata 1, 1: Kazusa DNA Research Institute, 2: Graduate School of Horticulture, Chiba University
Reference Ara T. et al. (2009) Plant Biotechnology 26(5): 445-449
Comment 和名:小糸在来、みすず

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Analytical Method Details Information

Title GC-TOF-MS lipid fraction method
Instrument 6890N GC (Agilent Technologies); Pegasus III TOF-MS system (Leco)
Instrument Type
Ionization EI
Ion Mode Positive
Description Frozen sample was homogenized using mill mixer with liquid nitrogen. Metabolites were extracted by MeOH/H2O/CHCl3. After liquid‐liquid distribution, upper (MeOH/H2O) layer is used as polar fraction and bottom (CHCl3) layer is used as lipid fraction. Ribitol and nonadecanoic acid methyl ester were used for internal standards of polar and lipid fractions. Lipid fraction was methoximated with CHCl3/NaOH/MeOH. After washing by water, CHCl3 layer was dried over by N2 gas. Metabolites were trimethylsilylated with n-Heptane/Pyridine/MSTFA. GC conditions: Agilent 6890N GC (Agilent), Column: DB-17ms (Agilent), Carrier gas: He, MS conditions: Pegasus III TOF-MS system (Leco), mass range: 82 to 500 m/z.
Comment_of_details Instrument Type: GC-TOF-MS

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