From Metabolonote
jump-to-nav Jump to: navigation, search

Sample Set Information

ID SE213
Title Comparison of metabolites among Lotus japonicus varieties
Description Investigation of Lotus japonicus metabolites. 2 varieties (B-129, MG-20) and 3 organs (leaf, stem, root) data are examined. The sample are analysed at Feb 2004.
Authors Takeshi Ara 1, Yoshihiko Morishita 1, Hideyuki Suzuki 1, Daisuke Shibata 1, 1: Kazusa DNA Research Institute
Reference Direct Submittion


Sample Information

ID S04
Title Lotus japonicus MG-20 Leaf
Organism - Scientific Name Lotus japonicus
Organism - ID NCBI taxonomy:34305
Compound - ID
Compound - Source
Sample Preparation Details ID

Analytical Method Information

ID M04
Title GC-TOF-MS analysis, polar fraction
Method Details ID MS2
Sample Amount
Comment [MassBase ID] MDGC1_04509

Link icon database.png Link icon massbase.png

The raw (binary) and near-raw (text) files of this analysis are available at MassBase.

Analytical Method Details Information

Title GC-TOF-MS polar fraction method
Instrument 6890N GC (Agilent Technologies); Pegasus III TOF-MS system (Leco)
Instrument Type
Ionization EI
Ion Mode Positive
Description Frozen sample was homogenized using mill mixer with liquid nitrogen. Metabolites were extracted by MeOH/H2O/CHCl3. After liquid‐liquid distribution, upper (MeOH/H2O) layer is used as polar fraction and bottom (CHCl3) layer is used as lipid fraction. Ribitol and nonadecanoic acid methyl ester were used for internal standards of polar and lipid fractions. Polar fraction was evaporated to dryness in a SpeedVac Concentrator. Metabolites were methoximated with methoxyamine hydrochloride/pyridine and then trimethylsilylated with MSTFA..GC conditions: Agilent 6890N GC (Agilent), Carrier gas: He, MS conditions: Pegasus III TOF-MS system (Leco), mass range: 35 to 950 m/z
Comment_of_details Instrument Type: GC-TOF-MS
Personal tools
View and Edit Metadata