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Sample Set Information

ID SE217
Title Comparison of tomato fruit metabolites among fruit maturation
Description Investigation of tomato fruit metabolites. 4 varieties (Solanum lycopersicum, Solanum lycopersicum cv. Micro-Tom, Solanum pimpinellifolium, Solanum lycopersicum x Solanum pimpinellifolium) and 4 maturation stages (green, mature green, breaker, red ripe)data are examined (May 2004).
Authors Takeshi Ara 1, Fumi Maeda 1, Hideyuki Suzuki 1, Daisuke Shibata 1, 1: Kazusa DNA Research Institute
Reference Direct submission


Sample Information

ID S01
Title Solanum lycopersicum cv. Micro-Tom Fruit breaker stage
Organism - Scientific Name Solanum lycopersicum
Organism - ID NCBI taxonomy:4081
Compound - ID
Compound - Source
Sample Preparation Details ID

Analytical Method Information

ID M12
Title GC-TOF-MS analysis, polar fraction
Method Details ID MS2
Sample Amount
Comment [MassBase ID] MDGC1_04226

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The raw (binary) and near-raw (text) files of this analysis are available at MassBase.

Analytical Method Details Information

Title GC-TOF-MS polar fraction method
Instrument 6890N GC (Agilent Technologies); Pegasus III TOF-MS system (Leco)
Instrument Type
Ionization EI
Ion Mode Positive
Description Frozen sample was homogenized using mill mixer with liquid nitrogen. Metabolites were extracted by MeOH/H2O/CHCl3. After liquid‐liquid distribution, upper (MeOH/H2O) layer is used as polar fraction and bottom (CHCl3) layer is used as lipid fraction. Ribitol and nonadecanoic acid methyl ester were used for internal standards of polar and lipid fractions. Polar fraction was evaporated to dryness in a SpeedVac Concentrator. Metabolites were methoximated with methoxyamine hydrochloride/pyridine and then trimethylsilylated with MSTFA..GC conditions: Agilent 6890N GC (Agilent), Carrier gas: He, MS conditions: Pegasus III TOF-MS system (Leco).
Comment_of_details Instrument Type: GC-TOF-MS
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