SE4:/MS5

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Sample Set Information

ID SE4
Title Dicksonia antarctica leaf metabolite analysis
Description Investigation of Dicksonia antarctica Leaf metabolites. 5 replicates data are examined.
Authors Takeshi Ara, Daisuke Nakajima, Mitsuo Enomoto, Nozomu Sakurai, Hideyuki Suzuki, Koei Okazaki, Daisuke Shibata, Kazusa DNA Research Institute
Reference Direct Submittion
Comment version 5


SE4 S01 200.jpg

Photo: Dicksonia antarctica (harvested sample (S01)) at Nambo Paradise Botanical Garden, Tateyama, Chiba, Japan (Feb 2008).

Analytical Method Details Information

ID MS5
Title LC-FT-ICR-MS ESI positive method 5
Instrument Agilent1100 HPLC (Agilent), LTQ-FT (Thermo Fisher Scientific)
Instrument Type LC-FTICR-MS
Ionization ESI
Ion Mode Positive
Description Harvested sample is frozen by liquid N2 and resulting powder (100mg) are solved in 300uL 80% methanol solution. The solution is dried up and is subjected to acid hydrolysis by 1.8% butanol/HCl, 95 degree C, 1hr. Butanol fraction is dried up and are solved in 300uL 80% methanol solution. The solution is subjected to Sep-Pak Light C18 cartridges (Waters). 20uL sample is injected into HPLC after 0.2um membrane filter treatment. HPLC conditions: Agilent 1100 series (Agilent), Column: TSKgel-100V (4.6 x 250 mm, 5μm; TOSOH), Solvent: A; 0.1% formic acid aq. B; ACN (addition 0.1% formic acid fc.), Gradient: (B);3 to 30% (0.0 to 20.0 min), 50 to 90% (20.0 to 35.0 min), 90% (35.0 to 45.0 min), 95% (45.1 to 50.0 min), 3% (50.1 to 57.0 min), Column temp.: 40 degree C, Flow rate=0.5mL/min, PDA: 200-650 nm (2 nm step). FT-ICR-MS conditions: Filter 1: FTMS + c norm !corona !pi res=100000 o(100.0-1500.0); 2: ITMS + c norm !corona !pi Dep MS/MS Most intense ion from (1); 3: ITMS + c norm !corona !pi Dep MS/MS 2nd most intense ion from (1); 4: ITMS + c norm !corona !pi Dep MS/MS 3rd most intense ion from (1); 5: ITMS + c norm !corona !pi Dep MS/MS 4th most intense ion from (1); 6: ITMS + c norm !corona !pi Dep MS/MS 5th most intense ion from (1)., Rejected mass=235.1800, 376.0400, 609.2800, 810.4100, 1123.5000
Comment_of_details


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