SE59:/S01
From Metabolonote
Sample Set Information
ID | SE59 |
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Title | Alternation of flavonoid accumulation under drought stress in Arabidopsis thaliana |
Description | We reported that flavonoids, a class of specialized metabolites, including flavonols and anthocyanins with strong radical scavenging activity contributed to the mitigation of oxidative and drought stress in Arabidopsis thaliana (Arabidopsis). However, the behavior of flavonoids during drought stress is still not well documented. Herein we investigated the time-series alternation of flavonoids in the aerial part of Arabidopsis (wild type, Col-0) during drought stress by LC-QTOF-MS. The drastic alternation of 5 flavonols and 5 anthocyanins was revealed together with changes in marker metabolites of drought stress, e.g., proline, raffinose, and galactinol. These findings indicate that flavonols and anthocyanins can mitigate drought stress. |
Authors | Ryo Nakabayashi, Tetsuya Mori, and Kazuki Saito |
Reference | Nakabayashi R et al. (2014) Plant Signaling & Behavior 9: e29518 |
Comment |
The raw data files are available at DROP Met web site in PRIMe database of RIKEN.
Sample Information
ID | S01 |
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Title | Arabidopsis |
Organism - Scientific Name | Arabidopsis thaliana |
Organism - ID | NCBI taxonomy:3702 |
Compound - ID | |
Compound - Source | |
Preparation | Treatment dose or intensity levels: The plants vertically grown in GM plates were incubated in 24-well plates (TPP, http://www.tpp.ch/index.php) and each plant was put in a well with 3 ml water.
Treatment time, time intervals and duration before harvest: 0 day Harvest date, time: no information Plant growth stage: 3 weeks (GM plate) + 0 day (treatment) Metabolism quenching method: Freezing with liquid N2 Harvest method: After removing root, the samples were immediately frozen with liquid nitrogen and lyophilized at −55 °C. Sample strage: The lyophilized materials were stored at −80 °C. Reprecate sampling and analyses: n=3 Tissue harvesting method: Time from tissue resection to liquid N2 freezing was one minute. Tissues were pooled in tube. |
Sample Preparation Details ID | SS01 |
Comment |
Sample Preparation Details Information
ID | SS01 |
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Title | Drought stress |
Description | Three-week-old plants vertically grown on GM plates were incubated in 24-well plates (TPP, http://www.tpp.ch/index.php). Each plant was put in a well with 3 ml water. Three-week-old plants were exposed to drought stress by stopping watering.The aerial samples were harvested at the time points of DAD 0, 3, and 5. |
Comment_of_details | DAD, days after drought. |