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Sample Set Information

Title LC-MS analysis of parsley
Description Comprehensive analysis of metabolites in parsley (Petroselium crispum, Paramount) was performed by several combination of sample preparation and analytical conditions.

Parsley was hydroponically grown in a greenhouse (S11-S13) or a cultivation shelf (S21-S26) using control medium and stable isotope (15N or 34S)-containing medium.

Authors Sakurai N, Suda K (Kazusa DNA Research Insitute)
Reference Akimoto N, Ara T, Nakajima D, Suda K, Ikeda C, Takahashi S, Muneto R, Yamada M, Suzuki H, Shibata D and Sakurai N (2017) FlavonoidSearch: A system for comprehensive flavonoid annotation by mass spectrometry. Scientific Reports 7: 1243

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Sample Information

ID S11
Title Shoot of greenhouse grown parsley
Organism - Scientific Name Petroselinum crispum, Paramount
Organism - ID NCBI taxonomy:4043
Compound - ID
Compound - Source
Preparation Hydroponically grown in a greenhouse. Shoot was harvested.
Sample Preparation Details ID SS1

Sample Preparation Details Information

Title Cultivation in a greenhouse
Description The parsley seeds (Kaneko Seeds, Co. LTD. KS100-542, Gunma, Japan) were germinated on watered sand. The 20 days-old seedlings were transplanted on baked foamed clay pebbles (Hydroball, Toshi Engei Col. LTD, Saitama, Japan) in a green house, and grown 63 days with culture medium containing 3 mM KNO3, 1 mM Ca(NO3)2, 0.5 mM MgSO4, 0.5 mM NH4H2PO4, 50 µM Fe-EDTA, 49 µM H3BO3, 9 µM MnSO4, 0.8 µM ZnSO4, 0.3 µM CuSO4, and 0.1 µM Na2MoO4. Shoot was harvested, ground to powder in liquid nitrogen by mortar and pestle, and stored at -80 ºC until use.
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