SE129:/S1
From Metabolonote
Sample Set Information
| ID | TSE9 |
|---|---|
| Title | MS-DIAL demo files |
| Description | Both data independent MS/MS acquisition (SWATH) and data dependent MS/MS acquisition (IDA) data sets is downloaded as the demo files of MS-DIAL. In order to use MS-DIAL program, the user has to convert the vendor's raw data to ABF file format. The demonstration for file convert can be performed via AB Sciex raw data sets (.wiff and .wiff.scan). The file converter is available at http://www.reifycs.com/english/AbfConverter/. If you want to demonstrate MS-DIAL itself, please use the converted files (.abf) from the below link. Also see http://prime.psc.riken.jp/Metabolomics_Software/MS-DIAL/MSDIAL%20quick%20start.pdf |
| Authors | Hiroshi Tsugawa, Tomas Cajka, Tobias Kind, Yan Ma, Brendan Higgins, Kazutaka Ikeda, Mitsuhiro Kanazawa, Jean VanderGheynst, Oliver Fiehn & Masanori Arita |
| Reference | Tsugawa et al. (2015) Nature Methods 12(6):523–526 |
| Comment |
The raw data files are available at DROP Met web site in PRIMe database of RIKEN.
Sample Information
| ID | S1 |
|---|---|
| Title | Chlamydomonas reinhardtii |
| Organism - Scientific Name | Chlamydomonas reinhardtii |
| Organism - ID | NCBI taxonomy 3055 |
| Compound - ID | |
| Compound - Source | |
| Preparation | The cultivation procedure of Chlamydomonas reinhardtii followed our previous report. The C. reinhardtii CC125 strain was streaked out from cryopreserved stock and cultivated in 75 mL TAP medium in 125 mL shake flasks at 25 °C under constant illumination with cool-white fluorescent bulbs at a fluence rate of 70 µmol m−2 s−1 and with continuous stirring (100 rpm). Four independent cultures were used for this study. The starter culture was harvested at late log-phase and 1 mL cell suspensions were then shifted to 75 mL of fresh TAP medium in 125 mL shake flasks. At 0.2–0.6 OD680 during the late-log phase, 1 mL cell suspensions were injected into 1 mL of –80 °C cold quenching solution composed of 70% methanol in water, centrifuged at 12,000 g for 2 min, and pellets were lyophilized and stored at −80 °C until further analysis. The same quenching procedure was used for all algae strains. |
| Sample Preparation Details ID | |
| Comment | Tsugawa et al. (2015) Nature Methods 12(6):523–526 |
