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Sample Set Information

ID TSE1332
Title Metabolome Analysis of Oryza sativa (Rice) Using Liquid ChromatographyMass Spectrometry for Characterizing Organ Specificity of Flavonoids with Anti-inflammatory and Anti-oxidant Activity
Description Oryza sativa L. (rice) is an important staple crop across the world. In the previous study, we identified 36 specialized (secondary) metabolites including 28 flavonoids. In the present study, a metabolome analysis using liquid chromatography-mass spectrometry was conducted on the leaf, bran, and brown and polished rice grains to better understand the distribution of these metabolites. Principal component analysis using the metabolome data clearly characterized the accumulation patterns of the metabolites. Flavonoids, e.g., tricin, tricin 7-O-rutinoside, and tricin 7-O-β-D-glucopyranoside, were mainly present in the leaf and bran but not in the polished grain. In addition, anti-inflammatory and anti-oxidant activity of the metabolites were assayed in vitro. Tricin 4'-O-(erythro-β-guaiacylglyceryl)ether and isoscoparin 2''-O-(6'''-(E)-feruloyl)-glucopyranoside showed the strongest activity for inhibiting nitric oxide (NO) production and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical-scavenging, respectively.
Authors Yang, Z., Nakabayashi, R., Mori, T., Takamatsu, S., Kitanaka, S. and Saito, K.
Reference Chem. Pharm. Bull. Volume 64 (2016) Issue 7 Pages 952-956

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Sample Information

ID S01
Title Oryza sativa L. (rice)
Organism - Scientific Name Oryza sativa L.
Organism - ID NCBI taxonomy:4530
Compound - ID
Compound - Source
Preparation The leaf samples (Habataki and Nipponbare cultivars) and the brown rice sample (Nipponbare cultivar) were harvested from the rice plants grown in our laboratory, as previously reported. The other brown rice samples (Nipponbare and Koshihikari cultivars), along with the bran and polished rice, were purchased from a local market. All samples were immediately frozen, lyophilized, ground, and stored at −80°C until required.
Sample Preparation Details ID

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Analytical Method Information

ID M01
Method Details ID MS01
Sample Amount 1 μL

Analytical Method Details Information

Instrument LC, Waters Acquity UPLC system; MS, Waters Xevo G2 Q-Tof
Instrument Type UPLC-QTOF-MS
Ionization ESI
Ion Mode positive and negative
Description The extraction and LC-QTOF-MS analysis was conducted as previously described.

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