SE213:/MS2

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Sample Set Information

ID SE213
Title Comparison of metabolites among Lotus japonicus varieties
Description Investigation of Lotus japonicus metabolites. 2 varieties (B-129, MG-20) and 3 organs (leaf, stem, root) data are examined. The sample are analysed at Feb 2004.
Authors Takeshi Ara 1, Yoshihiko Morishita 1, Hideyuki Suzuki 1, Daisuke Shibata 1, 1: Kazusa DNA Research Institute
Reference Direct Submittion
Comment


[PGDBj] http://pgdbj.jp/plantdb/plantinfo.html?ln=en&cmd=entry&ppid=t34305

Analytical Method Details Information

ID MS2
Title GC-TOF-MS polar fraction method
Instrument 6890N GC (Agilent Technologies); Pegasus III TOF-MS system (Leco)
Instrument Type
Ionization EI
Ion Mode Positive
Description Frozen sample was homogenized using mill mixer with liquid nitrogen. Metabolites were extracted by MeOH/H2O/CHCl3. After liquid‐liquid distribution, upper (MeOH/H2O) layer is used as polar fraction and bottom (CHCl3) layer is used as lipid fraction. Ribitol and nonadecanoic acid methyl ester were used for internal standards of polar and lipid fractions. Polar fraction was evaporated to dryness in a SpeedVac Concentrator. Metabolites were methoximated with methoxyamine hydrochloride/pyridine and then trimethylsilylated with MSTFA..GC conditions: Agilent 6890N GC (Agilent), Carrier gas: He, MS conditions: Pegasus III TOF-MS system (Leco), mass range: 35 to 950 m/z
Comment_of_details Instrument Type: GC-TOF-MS


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