SE213:/MS2
From Metabolonote
Sample Set Information
| ID | SE213 |
|---|---|
| Title | Comparison of metabolites among Lotus japonicus varieties |
| Description | Investigation of Lotus japonicus metabolites. 2 varieties (B-129, MG-20) and 3 organs (leaf, stem, root) data are examined. The sample are analysed at Feb 2004. |
| Authors | Takeshi Ara 1, Yoshihiko Morishita 1, Hideyuki Suzuki 1, Daisuke Shibata 1, 1: Kazusa DNA Research Institute |
| Reference | Direct Submittion |
| Comment |
Analytical Method Details Information
| ID | MS2 |
|---|---|
| Title | GC-TOF-MS polar fraction method |
| Instrument | 6890N GC (Agilent Technologies); Pegasus III TOF-MS system (Leco) |
| Instrument Type | |
| Ionization | EI |
| Ion Mode | Positive |
| Description | Frozen sample was homogenized using mill mixer with liquid nitrogen. Metabolites were extracted by MeOH/H2O/CHCl3. After liquid‐liquid distribution, upper (MeOH/H2O) layer is used as polar fraction and bottom (CHCl3) layer is used as lipid fraction. Ribitol and nonadecanoic acid methyl ester were used for internal standards of polar and lipid fractions. Polar fraction was evaporated to dryness in a SpeedVac Concentrator. Metabolites were methoximated with methoxyamine hydrochloride/pyridine and then trimethylsilylated with MSTFA..GC conditions: Agilent 6890N GC (Agilent), Carrier gas: He, MS conditions: Pegasus III TOF-MS system (Leco), mass range: 35 to 950 m/z |
| Comment_of_details | Instrument Type: GC-TOF-MS |
