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Title Metabolic profiling of flavonoids in Lotus japonicus
Description Metabolic profiling of flavonoids in leaf, stem, flower of Lotus japonicus Miyakojima MG-20 and Gifu B-129.
Authors Hideyuki Suzuki 1, Ryosuke Sasaki 1, Yoshiyuki Ogata 1, Yukiko Nakamura 2 4, Nozomu Sakurai 1, Mariko Kitajima 3, Hiromitsu Takayama 3, Shigehiko Kanaya 2, Koh Aoki 1, Daisuke Shibata 1, Kazuki Saito 3, 1: Kazusa DNA Research Institute, 2: Nara Institute of Science and Technology, 3: hiba University, 4: Ehime Women’s College
Reference Phytochemistry, 2008, 69(1), 99-111
Comment Seed coats of L. japonicus were scratched by glass paper and fed water for 1 d. The seedlings were transferred to a mixture of vermiculite and a commercial soil, Powersoil (mix ratio 1.3–1, Kureha Chemical Ind., Tokyo, Japan, and Kanto Hiryou Ind., Saitama, Japan), and grown for 90–120 days (from March to June) in 2005, in a greenhouse under natural sunlight. From 7-day-old plants, cotyledons and hypocotyls were collected separately and served as leaf and stem samples, respectively. From 14-, 21-, 30-, 60, and 90-day-old plants, leaves and stems were collected. The tissues for one experimental replicate were collected from 12 independent plants for 7-, 14-, and 21-day-old stages, or from one independent plant for 30-, 60-, and 90-day-old stages.

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The web resources and information related to the species used in this study are available at Plant Genome Database Japan (PGDBj).

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