SE42:/S02

Sample Set Information

ID	SE42
Title	Metabolic profiling of flavonoids in Lotus japonicus
Description	Metabolic profiling of flavonoids in leaf, stem, flower of Lotus japonicus Miyakojima MG-20 and Gifu B-129.
Authors	Hideyuki Suzuki 1, Ryosuke Sasaki 1, Yoshiyuki Ogata 1, Yukiko Nakamura 2 4, Nozomu Sakurai 1, Mariko Kitajima 3, Hiromitsu Takayama 3, Shigehiko Kanaya 2, Koh Aoki 1, Daisuke Shibata 1, Kazuki Saito 3, 1: Kazusa DNA Research Institute, 2: Nara Institute of Science and Technology, 3: hiba University, 4: Ehime Women’s College
Reference	Phytochemistry, 2008, 69(1), 99-111
Comment	Seed coats of L. japonicus were scratched by glass paper and fed water for 1 d. The seedlings were transferred to a mixture of vermiculite and a commercial soil, Powersoil (mix ratio 1.3–1, Kureha Chemical Ind., Tokyo, Japan, and Kanto Hiryou Ind., Saitama, Japan), and grown for 90–120 days (from March to June) in 2005, in a greenhouse under natural sunlight. From 7-day-old plants, cotyledons and hypocotyls were collected separately and served as leaf and stem samples, respectively. From 14-, 21-, 30-, 60, and 90-day-old plants, leaves and stems were collected. The tissues for one experimental replicate were collected from 12 independent plants for 7-, 14-, and 21-day-old stages, or from one independent plant for 30-, 60-, and 90-day-old stages.

The web resources and information related to the species used in this study are available at Plant Genome Database Japan (PGDBj).

http://pgdbj.jp/plantdb/plantinfo.html?ln=en&cmd=entry&ppid=t34305

PubMed

Sample Information

ID	S02
Title	Lotus japonicus B-129 leaf
Organism - Scientific Name	Lotus japonicus
Organism - ID	NCBI taxonomy:34305
Compound - ID
Compound - Source
Preparation
Sample Preparation Details ID	SS1
Comment

Sample Preparation Details Information

ID	SS1
Title	Growth condition
Description	Seed coats of L. japonicus were scratched by glass paper and fed water for 1 d. The seedlings were transferred to a mixture of vermiculite and a commercial soil, Powersoil (mix ratio 1.3–1, Kureha Chemical Ind., Tokyo, Japan, and Kanto Hiryou Ind., Saitama, Japan), and grown for 90–120 days (from March to June) in 2005, in a greenhouse under natural sunlight. From 7-day-old plants, cotyledons and hypocotyls were collected separately and served as leaf and stem samples, respectively. From 14-, 21-, 30-, 60, and 90-day-old plants, leaves and stems were collected. The tissues for one experimental replicate were collected from 12 independent plants for 7-, 14-, and 21-day-old stages, or from one independent plant for 30-, 60-, and 90-day-old stages.
Comment_of_details