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MS Description Sixteen plants were planted on a single pl Sixteen plants were planted on a single plate separated into fourths to minimize the differences in growth conditions. Four wild-type plants were planted on one-fourth, and four bsas mutant plants were planted on each of the remaining fourths. Five plates were replicated for each bsas mutant. Each sample was extracted with a concentration of 25 mg fresh weight of tissues per microliter of the extraction medium (methanol:chloroform:water [3:1:1; v/v/v]) by using a Retsh mixer mill MM 310 at a frequency of 30 Hz−1 for 3 min at 4°C. After centrifugation for 5 min at 15,100g, 400 μL of the supernatant of each plate were put together in accordance with each section of fourths. Four hundred microliters of the 2-mL supernatant were used for GC-TOF/MS analysis, and another 400 μL were used for CE-TOF/MS analysis.<br /><br /> The analysis of metabolites by GC-TOF/MS, including the derivatization step and the processing of MS data, was performed as described elsewhere (Kusano et al., 2007a, 2007b). d elsewhere (Kusano et al., 2007a, 2007b).
MS ID MS1  +
MS Instrument GC:Agilent 6890N MS:LECO Pegasus 4D MS system  +
MS Ion Mode Positive  +
MS Ionization EI  +
MS Title GC-TOF MS  +
Modification dateThis property is a special property in this wiki. 17 April 2018 01:27:09  +
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