| S Preparation
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Arabidopsis thaliana accession Columbia-0 … Arabidopsis thaliana accession Columbia-0 (Col-0; Lehle Seeds, http://www.arabidopsis.com/) was used as the wild-type in this study unless otherwise specified. The Arabidopsis TILLING lines CS88114, CS93994 and CS95581 for UGT79B6 (ugt79b6-1, ugt79b6-2 and ugt79b6-3, respectively), were obtained from the Arabidopsis Biological Resource Center (https://abrc.osu.edu/). The TILLING mutants are in the Col er105 (Big Mama) background (Torii et al., 1996). The services of the Seattle Tilling Project (http://tilling.fhcrc.org/) were used to screen for lines with point mutations in the coding region of UGT79B6. Specific primers for UGT79B6 (UGT79B6 left and UGT79B6 right), yielding a 1274 bp fragment starting 16 bp upstream from the first ATG, were used for screening. Homozygous knockout lines were screened by PCR using primers At5g54010(1f) and At5g54010(1362r). PCR products were sequenced to identify homozygous knockout lines using primer At5g54010(1258r). For phenotypic analyses, we used mutant lines back-crossed with Col–0 for three generations. -crossed with Col–0 for three generations.
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