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The pap1-D mutant of A. thaliana was descr … The pap1-D mutant of A. thaliana was described previously (Borevitz et al. 2000). The PAP1 cDNA-over-expressing transgenic A. thaliana (PAP1OX) was obtained by transformation with the construct carrying cauliflower mosaicvirus 35S promoter linked with the coding sequence of PAP1 cDNA as described elsewhere (Tohge et al. 2005). The transgenic plant expressing PAP1 chimeric repressor (PAP1SRDX) was described previously (Hiratsu et al. 2003, Matsui et al. 2004). The plants were grown in a standard greenhouse at 22 C in 16 h light for 8 weeks. Seeds were harvested and stored at room temperature for more than 1 week for drying. These seeds were used for analysis of anthocyanins, PAs, and radical-scavenging activity. ins, PAs, and radical-scavenging activity.
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