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MS Description Analysis was performed using samples with Analysis was performed using samples with three or four biological replicates per cultivar. Frozen rice tissue was homogenized in five volumes of cold 80% aqueous methanol containing an internal standard (0.5 mgL-1 lidocaine, Tokyo Kasei, Tokyo, Japan,, using a mixer mill (MM 300, Retsch, Haan, Germany, and a zirconia bead for 6 min at 20 Hz. Samples were centrifuged at 15 000 g for 10 min. The supernatant (3 μl) were subsequently subjected to metabolome analysis using liquid chromatography coupled with electrospray quadrupole time-of-flight tandem mass spectrometry with an Acquity BEH ODS column (LC-ESI-QToF/MS, HPLC: Waters Acquity UPLC system; MS: Waters QToF Premier, Metabolome analysis and data processing were conducted according to a previously described method (Matsuda et al., 2009, 2010). Briefly, metabolome data were obtained in positive ion mode (m/z 100–2000; dwell time: 0.5 sec), from which a data matrix was generated by MetAlign2 (Lommen and Kools, 2012). Signal intensities were normalized by dividing them by the intensities of the internal standard (lidocaine). A data matrix containing the 342 metabolite intensities from 668 runs was produced for the Japanese rice population (Tables S2 and S3). panese rice population (Tables S2 and S3).
MS ID MS01  +
MS Instrument Waters Acquity UPLC system and Waters Q-Tof Premier  +
MS Instrument Type UPLC-QTOF-MS  +
MS Ion Mode Positive  +
MS Ionization ESI  +
MS Title Metabolic profiling analysis using LC-ESI-Q-Tof/MS  +
Modification dateThis property is a special property in this wiki. 16 April 2018 05:23:44  +
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