SE210:/S03/M03
From Metabolonote
Sample Set Information
| ID | SE210 |
|---|---|
| Title | Comparison of seed metabolites among soybean varieties |
| Description | Investigation of Glycine max seed metabolites. 3 cultivers (Koitozairai, Masshoku, Misuzu) and Glycine max subsp. soja and 7 or 8 replicates data are examined. |
| Authors | Takeshi Ara 1, Yoshihiko Morishita 1, Hideyuki Suzuki 1, Kyuya Harada 2, Daisuke Shibata 1, 1: Kazusa DNA Research Institute, 2: Graduate School of Horticulture, Chiba University |
| Reference | Ara T. et al. (2009) Plant Biotechnology 26(5): 445-449 |
| Comment | 和名：小糸在来、みすず |
Sample Information
| ID | S03 |
|---|---|
| Title | Glycine max Misuzu Seed |
| Organism - Scientific Name | Glycine max |
| Organism - ID | NCBI taxonomy:3847 |
| Compound - ID | |
| Compound - Source | |
| Preparation | |
| Sample Preparation Details ID | |
| Comment |
Analytical Method Information
| ID | M03 |
|---|---|
| Title | GC-TOF-MS analysis, lipid fraction |
| Method Details ID | MS1 |
| Sample Amount | |
| Comment | [MassBase ID] MDGC1_02441 |
The raw (binary) and near-raw (text) files of this analysis are available at MassBase.
Analytical Method Details Information
| ID | MS1 |
|---|---|
| Title | GC-TOF-MS lipid fraction method |
| Instrument | 6890N GC (Agilent Technologies); Pegasus III TOF-MS system (Leco) |
| Instrument Type | |
| Ionization | EI |
| Ion Mode | Positive |
| Description | Frozen sample was homogenized using mill mixer with liquid nitrogen. Metabolites were extracted by MeOH/H2O/CHCl3. After liquid‐liquid distribution, upper (MeOH/H2O) layer is used as polar fraction and bottom (CHCl3) layer is used as lipid fraction. Ribitol and nonadecanoic acid methyl ester were used for internal standards of polar and lipid fractions. Lipid fraction was methoximated with CHCl3/NaOH/MeOH. After washing by water, CHCl3 layer was dried over by N2 gas. Metabolites were trimethylsilylated with n-Heptane/Pyridine/MSTFA. GC conditions: Agilent 6890N GC (Agilent), Column: DB-17ms (Agilent), Carrier gas: He, MS conditions: Pegasus III TOF-MS system (Leco), mass range: 82 to 500 m/z. |
| Comment_of_details | Instrument Type: GC-TOF-MS |
