SE213:/S05/M06

Sample Set Information

ID	SE213
Title	Comparison of metabolites among Lotus japonicus varieties
Description	Investigation of Lotus japonicus metabolites. 2 varieties (B-129, MG-20) and 3 organs (leaf, stem, root) data are examined. The sample are analysed at Feb 2004.
Authors	Takeshi Ara 1, Yoshihiko Morishita 1, Hideyuki Suzuki 1, Daisuke Shibata 1, 1: Kazusa DNA Research Institute
Reference	Direct Submittion
Comment

[PGDBj] http://pgdbj.jp/plantdb/plantinfo.html?ln=en&cmd=entry&ppid=t34305

Sample Information

ID	S05
Title	Lotus japonicus MG-20 Root
Organism - Scientific Name	Lotus japonicus
Organism - ID	NCBI taxonomy:34305
Compound - ID
Compound - Source
Preparation
Sample Preparation Details ID
Comment

Analytical Method Information

ID	M06
Title	GC-TOF-MS analysis, polar fraction
Method Details ID	MS2
Sample Amount
Comment	[MassBase ID] MDGC1_04518

The raw (binary) and near-raw (text) files of this analysis are available at MassBase.

Analytical Method Details Information

ID	MS2
Title	GC-TOF-MS polar fraction method
Instrument	6890N GC (Agilent Technologies); Pegasus III TOF-MS system (Leco)
Instrument Type
Ionization	EI
Ion Mode	Positive
Description	Frozen sample was homogenized using mill mixer with liquid nitrogen. Metabolites were extracted by MeOH/H2O/CHCl3. After liquid‐liquid distribution, upper (MeOH/H2O) layer is used as polar fraction and bottom (CHCl3) layer is used as lipid fraction. Ribitol and nonadecanoic acid methyl ester were used for internal standards of polar and lipid fractions. Polar fraction was evaporated to dryness in a SpeedVac Concentrator. Metabolites were methoximated with methoxyamine hydrochloride/pyridine and then trimethylsilylated with MSTFA..GC conditions: Agilent 6890N GC (Agilent), Carrier gas: He, MS conditions: Pegasus III TOF-MS system (Leco), mass range: 35 to 950 m/z
Comment_of_details	Instrument Type: GC-TOF-MS