SE215:/S05/M08

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Sample Set Information

ID SE215
Title Comparison of metabolites among Legume species
Description Investigation of Legume metabolites. 2 species (Medicago sativa, Medicago truncatula) and 3 varieties (B-129, Burttii, MG-20) of 1 species (Lotus japonicus) data are examined (Mar 2004).
Authors Takeshi Ara 1, Yoshihiko Morishita 1, Hideyuki Suzuki 1, Daisuke Shibata 1, 1: Kazusa DNA Research Institute
Reference Direct submission
Comment [PGDBj] http://pgdbj.jp/plantdb/plantinfo.html?ln=en&cmd=entry&ppid=t34305

[PGDBj] http://pgdbj.jp/plantdb/plantinfo.html?ln=en&cmd=entry&ppid=t3880

Sample Information

ID S05
Title Medicago truncatula
Organism - Scientific Name Medicago truncatula
Organism - ID NCBI taxonomy:3880
Compound - ID
Compound - Source
Preparation
Sample Preparation Details ID
Comment

Analytical Method Information

ID M08
Title GC-TOF-MS analysis, polar fraction
Method Details ID MS2
Sample Amount
Comment [MassBase ID] MDGC1_04800


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The raw (binary) and near-raw (text) files of this analysis are available at MassBase.

Analytical Method Details Information

ID MS2
Title GC-TOF-MS polar fraction method
Instrument 6890N GC (Agilent Technologies); Pegasus III TOF-MS system (Leco)
Instrument Type
Ionization EI
Ion Mode Positive
Description Frozen sample was homogenized using mill mixer with liquid nitrogen. Metabolites were extracted by MeOH/H2O/CHCl3. After liquid‐liquid distribution, upper (MeOH/H2O) layer is used as polar fraction and bottom (CHCl3) layer is used as lipid fraction. Ribitol and nonadecanoic acid methyl ester were used for internal standards of polar and lipid fractions. Polar fraction was evaporated to dryness in a SpeedVac Concentrator. Metabolites were methoximated with methoxyamine hydrochloride/pyridine and then trimethylsilylated with MSTFA..GC conditions: Agilent 6890N GC (Agilent), Carrier gas: He, MS conditions: Pegasus III TOF-MS system (Leco), mass range: 35 to 950 m/z.
Comment_of_details Instrument Type: GC-TOF-MS
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