SE54:/SS01
From Metabolonote
Sample Set Information
ID | SE54 |
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Title | Toward genome-wide metabolotyping and elucidation of metabolic system: metabolic profiling of large-scale bioresources |
Description | We established a novel methodology, termed widely targeted metabolomics, which can generate thousands of metabolome data points in a high-throughput manner. We previously conducted a targeted metabolite analysis of large-scale Arabidopsis bioresources, namely transposon-tagged mutants and accessions, to make a smaller dataset of metabolite accumulation. In this paper, we release approximately 3,000 metabolic profiles obtained by targeted analysis for 36 metabolites and discuss the possible regulation of amino acid accumulation. |
Authors | Masami Yokota Hirai, Yuji Sawada, Shigehiko Kanaya, Takashi Kuromori, Masatomo Kobayashi, Romy Klausnitzer, Kosuke Hanada, Kenji Akiyama, Tetsuya Sakurai, Kazuki Saito, Kazuo Shinozaki |
Reference | Hirai YM et al. (2010) Journal of Plant Research 123: 291-298 |
Comment |
The raw data files are available at DROP Met web site in PRIMe database of RIKEN.
Sample Preparation Details Information
ID | SS01 |
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Title | Sample Preparation |
Description | Seeds of 225 accessions of Arabidopsis were also analyzed for metabolic profiling. Seeds of each accession were sown on soil and germinated plants were grown in a greenhouse under natural light at 22℃ with application of 2,000-fold-diluted Hyponex until flowering finished. Then seeds were desiccated on plants for 1.5–2 months, harvested, and then stored at 4℃ and 20% humidity until use. |
Comment_of_details |