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Sample Set Information

ID TSE1307
Title Induced accumulation of glucuronosyldiacylglycerol in tomato and soybean under phosphorus deprivation.
Description Glucuronosyldiacylglycerol (GlcADG) is a plant glycolipid that accumulates in Arabidopsis and rice in response to phosphorus (P) starvation. It has been suggested that GlcADG functions to mitigate the stress induced by P depletion. Biosynthesis of GlcADG requires sulfolipid (SQDG) synthase, which is coded for in plant genomes. This indicates the possibility that GlcADG may be a general constituent of membrane lipids in plants. In this study, we investigated the SQDG synthases found in the genomes of higher plants, ferns, mosses, algae and cyanobacteria. In addition, we analyzed GlcADG accumulation, and the expression of SQDG synthase homologs in tomato and soybean plants grown under P-limited conditions. LC-MS analysis of lipids from these plants confirmed that GlcADG accumulated during P deprivation, as previously observed in Arabidopsis and rice. We also observed upregulation of SQDG synthase transcripts in these plants during P deprivation. These data suggest that GlcADG is present not only in model plants, but also in various other plant species, and that this lipid molecule performs an important physiological function as a mitigator of P-deprivation stress in plants.
Authors Okazaki Y, Nishizawa T, Takano K, Ohnishi M, Mimura T, Saito K.
Reference Physiol Plant. 2015 Sep;155(1):33-42. doi: 10.1111/ppl.12334. Epub 2015 Mar 12.

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Sample Information

Title Tomato
Organism - Scientific Name Solanum lycopersicum
Organism - ID NCBI taxonomy:4081
Compound - ID
Compound - Source
Preparation Tomato seeds (Solanum lycopersicum cv. Moneymaker) were sterilized by washing with 70% ethanol, followed by gentle agitation in 2% (v/v) plant preserve mixture (PPM; Plant Cell Technology, Jefferson, Washington, D.C., USA) in the dark, overnight, and at room temperature.

Sterilized seeds were incubated for 10 days on wet filter paper at 22°C (18-h light phase/6-h dark phase). Ten-day-old plants were transplanted to rockwool (Grodan) and grown in liquid MGRL medium (Fujiwara et al. 1992). For the P starvation treatment, plants were grown in MGRL medium without potassium phosphate.

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