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Sample Set Information

Title Toward genome-wide metabolotyping and elucidation of metabolic system: metabolic profiling of large-scale bioresources
Description We established a novel methodology, termed widely targeted metabolomics, which can generate thousands of metabolome data points in a high-throughput manner. We previously conducted a targeted metabolite analysis of large-scale Arabidopsis bioresources, namely transposon-tagged mutants and accessions, to make a smaller dataset of metabolite accumulation. In this paper, we release approximately 3,000 metabolic profiles obtained by targeted analysis for 36 metabolites and discuss the possible regulation of amino acid accumulation.
Authors Masami Yokota Hirai, Yuji Sawada, Shigehiko Kanaya, Takashi Kuromori, Masatomo Kobayashi, Romy Klausnitzer, Kosuke Hanada, Kenji Akiyama, Tetsuya Sakurai, Kazuki Saito, Kazuo Shinozaki
Reference Hirai YM et al. (2010) Journal of Plant Research 123: 291-298

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The raw data files are available at DROP Met web site in PRIMe database of RIKEN.

Sample Preparation Details Information

Title Sample Preparation
Description Seeds of 225 accessions of Arabidopsis were also analyzed for metabolic profiling. Seeds of each accession were sown on soil and germinated plants were grown in a greenhouse under natural light at 22℃ with application of 2,000-fold-diluted Hyponex until flowering finished. Then seeds were desiccated on plants for 1.5–2 months, harvested, and then stored at 4℃ and 20% humidity until use.

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